Dot blot hybridisation of RNA - Molecular Biology
Dot blot hybridisation of RNA - Molecular Biology
nylon membranes using a slot blot apparatus The membranes were baked, hybridized to the AAV probe, washed, and autoradiographed at –70 C for 90 min The
Slot or dot blotting is a technique whereby nucleic acids can be applied to a solid matrix, unfractionated, using a vacuum manifold Slot blotting is the Slot Blot Apparatus: A slot blot apparatus, which typically consists of a platform with slots or wells, is used Each slot corresponds to a
aluminium t slot channel By following the important points mentioned above, candidates can ensure a smooth and hassle-free counselling process ,The slot blot apparatus No dot-blot manifold is necessary, only a modified 96-well flat-bottom microtiter plate This apparatus is re-usable, inexpensive and especially suited for
