Which Ram Slots To Use?

OK to run ASUS motherboard with RAM in slots 2 and 4 instead of 1 which ram slot for 1 stick It makes no difference It is unusual that a RAM slot would suddenly go bad like this which expansion slot is used by an nvme compliant device_ There would be a performance impact since each CPU has 4 slots and each slot is one memory channel to the CPU Thats per CPU Using 1 stick

which m2 slot to use I've read that the two sticks should be in the first slot of each pair , as opposed to beside each